Galene

Galene is a tool to correct for sample motion in laser scanning fluorescence microscopy data, supporting both intensity-only and fluorescence lifetime imaging (FLIM) data.

Using an approach based on the Lucas–Kanade framework, Galene estimates the sample displacement during each frame relative to a reference frame. Using this information, the FLIM image may be reconstructed accounting for this motion, reassigning each photon arrival to the correct pixel producing a distortion-free image. For more information please see Warren et al. 2017

This documentation will help you get started using Galene. If you use Galene in your paper, please cite us

• Getting started
  • System Requirements
  • Installing on Windows
  • Installing on macOS
• Acquiring data
  • Configuring your microscope
    • Picoquant
    • Becker and Hickl
  • Optimal scanner parameters
• Tutorial
  • Loading and opening data
  • Correcting for motion
  • Viewing the realignment results
  • Optimising the realignment results
  • Saving the realigned data
• Correction Modes
  • Translation
  • Warp
• Citing Galene